Pinewoods Conservation Group has submitted their objection to plans for 124 houses to be built on the edge of the historic woodland.
The group have raised concerned regarding the ecological plans for the site and say that a protected species of bats could be put at risk if the house building goes ahead.
Taylor Wimpey submitted plans last month for 124 dwellings to be built on green space at Crag lane off Otley Road, adjacent to The Pinewoods and opposite RHS Harlow Carr.
Secretary Michelle Whitfield-Speed said: “After careful consideration of the plans, and with a direct impact on The Pinewoods we had no option but to submit a formal objection.
“There is clear evidence that the surrounding environment and wildlife will be severely impacted should this planning be approved.
“The land in question is not earmarked for development in the Local Development Framework and is designated as a special landscape area.”
Murray Trantor, chairman of Harlow and Pannal Ash Residents Association has also opposed the plans.
In a letter to the council he said: “This planning application reverts to essentially the same plan that we have opposed for the last five years. This plan enjoys absolutely no local support, not only from people living in the west of Harrogate but from the whole community.”
View the plans on Harrogate council’s planning site ref: 14/00259/OUTMAJ.
Harrogate residents who are concerned about this development and the future conservation of The Pinewoods are encouraged to join the Pinewoods Conservation Group via http://pinewoodsconservationgroup.org.uk/how-join.