The closure is necessary while Fort Bridge is demolished as part of the Leeming to Barton major road scheme.
It will shut on Saturday at 8pm and will remain closed until 6am on Monday November 16 in both directions between Junction 51 Leeming Bar and Scotch Corner.
The bridge needs to be demolished to allow space for an extra lane to be added to the road network before being rebuilt at a later date.
In the meantime drivers will be able to use a new Catterick Central junction, which is now open and located north of the existing junction.
“This is a significant milestone for the scheme and a complex piece of engineering, which is why it is essential we close sections of the A1," said Highways England project manager Tom Howard.
“We will use 35-tonne excavators and are building a platform to accommodate a 500-tonne crane to remove the steel beams from the existing bridge structure.
“There will inevitably be delays at peak times and people should plan their journeys, using the many alternative routes that will be signposted off the A1 closure and leave extra time to complete their journeys. We would like to thank drivers for their patience.”
Clearly signed diversions will be in place and drivers are advised to allow an extra 30 minutes to their journey time.
The £380 million Highways England project, which began last year, will replace the existing dual carriageway with a new three-lane motorway.
When completed the A1 Leeming to Barton scheme will create a continuous motorway-standard route between London and Newcastle (including the M1 and A1M). It will also improve journey times and increase safety.