The Harrogate branch of Lewis & Cooper has shut the doors of its Parliament Street store.
Gourmet food and wine store Lewis & Cooper had only been trading in Harrogate since December 2011.
The lease on the store was coming to an end, and a Lewis & Cooper statement said it could not justify relocating.
A statement read: “Sadly, closed our doors at Harrogate store for the last time.
“Thanks to all our hardworking staff and lovely loyal customers.
“Landlord has other plans for building and sales figures, while promising, didn’t justify a relocation to new premises.”
Lewis & Cooper will remain open in Northallerton, where it has been trading since 1899.
Twitter users expressed their upset online, in a week of worrying news for the High Street.
In response to Lewis & Cooper’s closure, Sammy M said: “Sorry to see the closure of your Parliament Street store. Bad news for Harrogate.”
Harrogate Tourist Info said: “Sad that Lewis and Cooper are closing down in Harrogate. Hope you can come back soon.”
Jobs are under threat at the Harrogate branches of Blockbuster, in Starbeck and Cold Bath Road, and HMV, on Cambridge Street.
Administrators Deloitte have announced 129 Blockbuster stores will shut down nationwide in the coming weeks.
It is unclear if the Harrogate stores will be affected.